Western blot abreview for Anti-Mitofusin 2 antibody

Good
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Pig Tissue lysate - whole (heart left ventricule)
Specification
heart left ventricule
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Mouse HRP Cell Signaling cat no:7076S
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Bands
Specific: 80-90 kDa Non-specific: 45-50, 250 kDa
Exposure
3 second(s)

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Submitted Oct 24 2014

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