Western blot abreview for Anti-Mitofusin 2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (Cell line: cytosolic fraction and mitochondrial fr)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Cell line: cytosolic fraction and mitochondrial fr
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 1%BSA
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-mouse IgG-HRP: sc-2031
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 75 kDa
Positive control
Samples fractionated to obtain Mitochondrial fraction and Cytosolic fraction.

Additional data

Additional Notes
Can be used also for IP.

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Submitted May 25 2015

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