Immunocytochemistry/ Immunofluorescence abreview for Anti-Mitofusin 2 antibody

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.1% Triton X100
Specification
HEK293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% donkey serum
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
Washing protocol: After primary and secondary antibody incubation steps cells were washed in 24 well plates, 4 x 10 minutes with 0.5 ml of 1x PBS, 0.1% Triton X100.

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Submitted Apr 11 2017

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