Key features and details
- Rabbit polyclonal to Mitofusin 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Mitofusin 2 antibody
See all Mitofusin 2 primary antibodies
DescriptionRabbit polyclonal to Mitofusin 2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cynomolgus monkey, Orangutan
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 84 kDa).|
FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
Tissue specificityUbiquitous; expressed at low level. Highly expressed in heart and kidney.
Involvement in diseaseCharcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A
Sequence similaritiesBelongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
modificationsPhosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
Cellular localizationMitochondrion outer membrane. Colocalizes with BAX during apoptosis.
- Information by UniProt
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
All lanes : Anti-Mitofusin 2 antibody (ab50843) at 0.25 µg/ml
Lane 1 : Rat brain mitochondria
Lane 2 : Mouse brain mitochondria
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate.
Predicted band size: 84 kDa
Observed band size: 86 kDa why is the actual band size different from the predicted?
ab50843 has been referenced in 8 publications.
- de-Lima-Júnior JC et al. Abnormal brown adipose tissue mitochondrial structure and function in IL10 deficiency. EBioMedicine 39:436-447 (2019). PubMed: 30502051
- Faitg J et al. Effects of Aging and Caloric Restriction on Fiber Type Composition, Mitochondrial Morphology and Dynamics in Rat Oxidative and Glycolytic Muscles. Front Physiol 10:420 (2019). PubMed: 31114501
- Vázquez-Fonseca L et al. ADCK2 Haploinsufficiency Reduces Mitochondrial Lipid Oxidation and Causes Myopathy Associated with CoQ Deficiency. J Clin Med 8:N/A (2019). PubMed: 31480808
- Huang S et al. MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin-2 in arteriosclerosis obliterans of the lower extremitie. Exp Ther Med 15:633-640 (2018). PubMed: 29403548
- Brownstein AJ et al. Heat stress causes dysfunctional autophagy in oxidative skeletal muscle. Physiol Rep 5:N/A (2017). WB . PubMed: 28646096
- Su L et al. Activation of transient receptor potential vanilloid 1 accelerates re-endothelialization and inhibits neointimal formation after vascular injury. J Vasc Surg 65:197-205.e2 (2017). PubMed: 26947234
- Ruiz LM et al. Quercetin Affects Erythropoiesis and Heart Mitochondrial Function in Mice. Oxid Med Cell Longev 2015:836301 (2015). WB ; Mouse . PubMed: 26106459
- Fahrenbach JP et al. Abcc9 is required for the transition to oxidative metabolism in the newborn heart. FASEB J 28:2804-15 (2014). PubMed: 24648545