Overview

  • Product name

    Anti-Mitofusin 2 antibody [N153/5]
    See all Mitofusin 2 primary antibodies
  • Description

    Mouse monoclonal [N153/5] to Mitofusin 2
  • Host species

    Mouse
  • Specificity

    ab186317 does not cross react with Mitofusin 1.
  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Fusion protein corresponding to Mouse Mitofusin 2 aa 370-600.
    Sequence:

    E AVRLIMDSLH IAAQEQRVYC LEMREERQDR LRFIDKQLEL LAQDYKLRIK QITEEVERQV STAMAEEIRR LSVLVDEYQM DFHPSPVVLK VYKNELHRHI EEGLGRNLSD RCSTAIASSL QTMQQDMIDG LKPLLPVSMR NQIDMLVPRQ CFSLSYDLNC DKLCADFQED IEFHFSLGWT MLVNRFLGPK NSRRALLGYS DQVQRPLPLT PANPSMPPLP QSSLTQEELM


    Database link: Q80U63

  • Positive control

    • WB: Rat brain membrane lysate; Mouse cardiac mitochondrial lysate.
  • General notes

    The clone number has been updated from S153-5 to N153/5, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab186317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 86 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
  • Tissue specificity

    Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
  • Involvement in disease

    Charcot-Marie-Tooth disease 2A2
    Neuropathy, hereditary motor and sensory, 6A
  • Sequence similarities

    Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
  • Post-translational
    modifications

    Phosphorylated by PINK1.
    Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
  • Cellular localization

    Mitochondrion outer membrane. Colocalizes with BAX during apoptosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMT2A antibody
    • CMT2A2 antibody
    • CPRP 1 antibody
    • CPRP1 antibody
    • EC 3.6.5.- antibody
    • Fzo antibody
    • HSG antibody
    • hyperplasia suppressor gene antibody
    • Hypertension related protein 1 antibody
    • KIAA0214 antibody
    • MARF antibody
    • MFN 2 antibody
    • Mfn2 antibody
    • MFN2_HUMAN antibody
    • Mitochondrial assembly regulatory factor antibody
    • Mitofusin-2 antibody
    • Mitofusin2 antibody
    • Transmembrane GTPase MFN2 antibody
    see all

Images

  • ab186317 staining Mitofusin 2 in the Human Neuroblastoma cell line (C) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with4% formaldehyde for 15 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. An ATTO 488-conjugated Goat anti-mouse polyclonal was used as the secondary antibody (1/100). Nucelus stained blue with DAPI (A), F-Actin stained red with Phalloidin Texas Red (B) and merged images (D)

  • Anti-Mitofusin 2 antibody [N153/5] (ab186317) at 1/200 dilution + Rat brain membrane lysate at 15 µg

    Predicted band size: 86 kDa

References

ab186317 has not yet been referenced specifically in any publications.

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