Key features and details
- Mouse monoclonal [N153/5] to Mitofusin 2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Product nameAnti-Mitofusin 2 antibody [N153/5]
See all Mitofusin 2 primary antibodies
DescriptionMouse monoclonal [N153/5] to Mitofusin 2
Specificityab186317 does not cross react with Mitofusin 1.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Fusion protein corresponding to Mouse Mitofusin 2 aa 370-600.
E AVRLIMDSLH IAAQEQRVYC LEMREERQDR LRFIDKQLEL LAQDYKLRIK QITEEVERQV STAMAEEIRR LSVLVDEYQM DFHPSPVVLK VYKNELHRHI EEGLGRNLSD RCSTAIASSL QTMQQDMIDG LKPLLPVSMR NQIDMLVPRQ CFSLSYDLNC DKLCADFQED IEFHFSLGWT MLVNRFLGPK NSRRALLGYS DQVQRPLPLT PANPSMPPLP QSSLTQEELM
Database link: Q80U63
- WB: Rat brain membrane lysate; Mouse cardiac mitochondrial lysate.
The clone number has been updated from S153-5 to N153/5, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 86 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
Tissue specificityUbiquitous; expressed at low level. Highly expressed in heart and kidney.
Involvement in diseaseCharcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A
Sequence similaritiesBelongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
modificationsPhosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
Cellular localizationMitochondrion outer membrane. Colocalizes with BAX during apoptosis.
- Information by UniProt
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
ab186317 staining Mitofusin 2 in the Human Neuroblastoma cell line (C) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with4% formaldehyde for 15 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. An ATTO 488-conjugated Goat anti-mouse polyclonal was used as the secondary antibody (1/100). Nucelus stained blue with DAPI (A), F-Actin stained red with Phalloidin Texas Red (B) and merged images (D)
Anti-Mitofusin 2 antibody [N153/5] (ab186317) at 1/200 dilution + Rat brain membrane lysate at 15 µg
Predicted band size: 86 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab186317 has not yet been referenced specifically in any publications.