FITC Anti-Mitofusin 2 antibody [N153/5] (ab186319)
Key features and details
- FITC Mouse monoclonal [N153/5] to Mitofusin 2
- Suitable for: IHC-Fr, IHC-P, ICC/IF, WB
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
FITC Anti-Mitofusin 2 antibody [N153/5]
See all Mitofusin 2 primary antibodies -
Description
FITC Mouse monoclonal [N153/5] to Mitofusin 2 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab186319 does not cross react with Mitofusin 1. -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Mouse Mitofusin 2 aa 370-600.
Database link: Q80U63 -
Positive control
- Mouse cardiac mitochondrial lysate.
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General notes
The clone number has been updated from S153-5 to N153/5, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N153/5 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. -
Tissue specificity
Ubiquitous; expressed at low level. Highly expressed in heart and kidney. -
Involvement in disease
Charcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A -
Sequence similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain. -
Post-translational
modificationsPhosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion. -
Cellular localization
Mitochondrion outer membrane. Colocalizes with BAX during apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 9927 Human
- Entrez Gene: 170731 Mouse
- Entrez Gene: 64476 Rat
- Omim: 608507 Human
- SwissProt: O95140 Human
- SwissProt: Q80U63 Mouse
- SwissProt: Q8R500 Rat
- Unigene: 376681 Human
see all -
Alternative names
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab186319 has not yet been referenced specifically in any publications.