Key features and details
- Mouse monoclonal [N153/5] to Mitofusin 2 (FITC)
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Product nameAnti-Mitofusin 2 antibody [N153/5] (FITC)
See all Mitofusin 2 primary antibodies
DescriptionMouse monoclonal [N153/5] to Mitofusin 2 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Specificityab186319 does not cross react with Mitofusin 1.
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
Recombinant fragment corresponding to Mouse Mitofusin 2 aa 370-600.
Database link: Q80U63
- Mouse cardiac mitochondrial lysate.
The clone number has been updated from S153-5 to N153/5, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186319 in the following tested applications.
|WB||1/1000. Predicted molecular weight: 86 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
Tissue specificityUbiquitous; expressed at low level. Highly expressed in heart and kidney.
Involvement in diseaseCharcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A
Sequence similaritiesBelongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
modificationsPhosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
Cellular localizationMitochondrion outer membrane. Colocalizes with BAX during apoptosis.
- Information by UniProt
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab186319 has not yet been referenced specifically in any publications.