Product nameMitomycin C (MMC), Anticancer and antibiotic agent
DescriptionAnticancer and antibiotic agent
Anticancer and antibiotic agent. Crosslinks DNA. Inhibits DNA synthesis and nuclear division. Also induces apoptosis.
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Soluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Functional assays: Caspase 8 (active) FITC Staining Kit (ab65614)
Active caspase 8 in control Jurkat cells (10e6/mL) or cells treated for five hours with 10 ug/mL Cyclohexamide (CHX) (ab120093) or four hours with 25 ug/mL Mitomycin C (MitoC) (ab120797). Background signal subtracted, duplicates; +/- SD.
This product has been referenced in:
- Mladenov E et al. Activation of the S phase DNA damage checkpoint by mitomycin C. J Cell Physiol 211:468-76 (2007). Read more (PubMed: 17167777) »
- Park IC et al. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner in human gastric adenocarcinoma cells. Cancer Lett 158:125-32 (2000). Read more (PubMed: 10960761) »
- Sasaki M et al. Re-evaluation of tumor-specific cytotoxicity of mitomycin C, bleomycin and peplomycin. Anticancer Res 26:3373-80 (0). Read more (PubMed: 17094455) »