Question (11406) | Anti-Mitotic Cells antibody [8B3G] (ab8956)

Go to datasheet (ab8956)

Question

The immunogen is stated as being "Total cell lysate of the human bladder carcinoma cell line T24." Are there more specifics regarding this? What specifically is the antibody binding to? Are there any references regarding this clone?

Answer

The mice that were used to produce ab8956 were indeed immunized with a total cell lysate of proliferating T24. Since the antibody behaves rather poorly in immunoblotting or the amount of its antigen in the cell is low, we have not been able to clearly identify the protein to which ab8956 binds. We have performed flow cytometric analyses with ab8956 that revealed a particularly strong reactivity in mitotic cells (see datasheet}. Unfortunately ab8956 has not yet been published with.

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