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Thank you very much for the detailed information. I admit that we did
not have too high expectations regarding this antibody in WB, however
because the results obtained in IHC-Fr were inconclusive (unspecific,
binding in many areas), I tried to see which kind, and how many
different antigens does this antibody recognize. As it looks like, it
recognized several bands, possibly signifying several very different
proteins - which certainly would explain the unspecific binding observed
Regarding the secondary antibody: indeed a different antibody was used:
goat polyclonal to mouse IgM - DyLight 488 (ab97007) Thank you for
catching this error in the Abreview! Below I have revised the Abreview
to reflect the correct sequence of antibodies:
Asked on Dec 06 2012
Thank you very much for your reply and providing this additional information. We appreciate the time that was spent providing this Abreview.
The Abreview has now been published and you have received the reward points.
Please do not hesitate to contact us with further questions.
Answered on Dec 06 2012