• Product name
    MitoTox™ Complex I OXPHOS Activity Assay Kit
    See all Complex I kits
  • Detection method
  • Sample type
    Inhibitor compounds
  • Assay type
  • Assay time
    5h 00m
  • Product overview

    MitoTox™ Complex I OXPHOS Activity Assay (ab109903) is designed for testing the direct inhibitory effect of compounds on Complex I activity in only 5 hours. Complex I extracted from the provided bovine heart mitochondria (a rich source of Complex I) is immunocaptured by specific antibodies on the plate. Complex I activity can be observed as decrease in absorbance at OD 340 nm. The intra-assay and inter-assay variation of this assay are both < 10%.

    Inhibitory effects of compounds on Complex I activity can be tested in two different ways: 1. Screening format, where up to 23 compounds can be tested at a single concentration in triplicate; 2. Dose response (IC50) format, where two compounds known to affect Complex I activity can be tested at 11 different data points in triplicate.

    Testing for mitochondrial function has become a key aspect of drug discovery. Mitochondria can be affected by drug treatment, resulting into cardio- and hepatotoxic side effects that can lead to drug withdrawal from the market. Therefore, there is increasing emphasis on testing the impact on mitochondria early on in the drug development process to reduce failure rates during preclinical and clinical phases. 

  • Notes

    Store Phospholipids, Bovine heart mitochondria, Ubiquinone and Activity Buffer at -80°C. Store all other components at 4°C.

  • Platform



  • Typical dose response curve for rotenone. Assay was performed following the Dose Response Assay Procedure using rotenone, a well known Complex I inhibitor. Rotenone was prepared in DMSO to generate a 10 mM stock. Starting with a 50 µM final concentration in well, 1:10 serial dilutions of rotenone were generated.



This product has been referenced in:
  • Cameron AR  et al. Metformin selectively targets redox control of complex I energy transduction. Redox Biol 14:187-197 (2018). Read more (PubMed: 28942196) »
  • Choi SE  et al. NF-?B/p53-activated inflammatory response involves in diquat-induced mitochondrial dysfunction and apoptosis. Environ Toxicol N/A:N/A (2018). Read more (PubMed: 29484840) »

See all 5 Publications for this product

Customer reviews and Q&As

The plate is one solid plate and not removable strips. Plate is best used at once as there is risk to unused wells getting wet, etc. We have not tried this and therefore cannot guarantee, but you could try putting a plate seal over...

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The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine

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Thank you for contacting us.

I have checked with our MitoSciences lab and they sent the attached QC data for the plates used in that lot. Additionally, these kits are designed to be run with developmental compounds. On occasion, we have seen ...

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Les phospholipides peuvent en effet être le paramètre critique pour le bon fonctionnement du kit MTOX I.Ils ne sont pas stables si ils sont conservés à 4ºC et doivent être impérativement stockés &agra...

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Je suis ravi que les suggestions aient été utiles.

J'ai transmis votre question au laboratoire et vous contacterai de nouveau dès que j'aurai reçu une réponse.

Merci de votre réponse.

Le laboratoire vient de m'indiquer que la valeur de laDO initiale mesurée lors des tests de contrôle qualité était de 1 pour le lot GR78475-2.

Le problème rencontr&eac...

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Merci de nous avoir contactés.

La DO initiale (0.5) est en effet un peu faible et pourrait expliquer la valeur anormalementélevée de l'IC50.

Une des raisons expliquant la faible DO est l'age du kit et les condit...

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Thank you for your enquiry.

The solvent for the Ubiquinone 1 component is 100% ethanol.

Thank you for your enquiry. Please find the answers to your questions below.

1) Thank you for your feedback. We will take this into consideration with the production team.

2) Typically customers have used this kit in combination w...

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Thank you for your enquiry and your interest in our products. ab109905 is the only assay which is not an immunoassay. The supplied bovine sample can be replaced with a mitochondrial sample from any other species. This may require some optimization ...

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1-10 of 17 Abreviews or Q&A


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