For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Hello ! I want to measure the mitochondria complex III activity using a kit . I noted that there is a product titled MitoTox™ Complex III OXPHOS Activity Microplate Assay (ab109905). And i see that this product is designed to quick screening drugs. Can I use this kit to measure the complex III activity if i use my own sample mitochondria such as rat mitochondria extracted from heart instead of provided mitochondria in the kit and the other procedure is still the same? Can the antibody precoated onto the plate react with rat species ? And about measure other complex activity kit ,for example, for complex I activity kit ,there are two products. one is Complex I Enzyme Activity Microplate Assay Kit (ab109721) , the other is MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903). I want to know what is the differences between this two kit. And what is the reason for there is only a kind of kit for complex III. why not have the activity kit for complex III like Complex I Enzyme Activity Microplate Assay Kit (ab109721) ? What is the difference between complex III and other complex ? Thank you very much. Urgently looking forward to your reply! Best regards! Qian L
Asked on Dec 28 2011
Thank you for your enquiry and your interest in our products. ab109905 is the only assay which is not an immunoassay. The supplied bovine sample can be replaced with a mitochondrial sample from any other species. This may require some optimization to identify a sample specific amount to use where the rate is acceptable. Fortunately, the kit is supplied with sufficient reagents to perform a test run to determine an acceptable rate. I hope this helps and if I can assist further, please do not hesitate to contact me.
Answered on Dec 28 2011