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i bought the kit as "ab109905" from your company and i assayed the inhibition effect of desire compound on the complex III activity via dose response assay.Im going toalculate the complex III activity but i faced a problem!whats the background rate (0.3 mOD/min) that has writenin the protocole?
please explain the calculation section for me step by step!
Asked on May 21 2012
Thank you for contacting us.
The 0.3 mOD/min is an example figure, your results may vary slightly. This background rate is the rate observed in your control wells that do not contain any mitochondria. The activity is measured by taking the average of your sample readings for a particular treatment, and subtracting the background rate. This activity measurement can then be used to compare different methods of treatment.
I hope this helps, if you have any further questions, please let me know and I will be happy to help you further.
Answered on May 21 2012