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What we are interested in is whether the assays are sensitive enough for our application.
Will the abpromise mean that we are able to obtain a refund on the product if it is not sensitive enough?
Asked on Aug 06 2012
Thank you for your reply.
The only data for sensitivity we have at the moment for ab112150 can be found on the datasheet as graph. I also include the actual numbers here:
NIR fluorescence, four replicates.
Control FCCP (20 uM)
This assay was not designed to measure the activity of BHM, but rather the effect of a compound on a robust activity of BHM (which is provided with the kit). Ifyou want to usewhole mitochondria,the amount of mitochondria will have to be optimized, so that before you test compounds, you can measure a robust activity. The kit should work with any species, however the quality of the mitos will be very important.
Are you asking forthe sensitivity of a particular drug on the activity of complex III (II+III)? This of course is compound dependant, but we can provide the IC50 for some examples we have run in house.
Are you asking for the sensitivity of the assay at detecting BHM? The assay does not have an antibody. This is an in-solution assay which measures complex II+III in a 96 well plate. The assay was designed to use a robust signal of BHM so that compounds can be tested. Also, the assay does not have a “control protein complex II+III” to be able to produce the exact numbers I understand you wnat to see. We provide the BHM sample (which contains all the complexes of the electron transport chain). When customers run their assay, they should run the assay in the presence of compound and diluents. The diluent will typically be DMSO, which does not affect the assay at concentrations of 1 – 2%.
Do youwant to know the raw signal in mOD/min in the presence of DMSO?. We can provide this as a reference guide, but not for ab109905, because the raw number is dependent on the BHM batch preparation and the substrates batch preparation. We can however giveout raw data from the QC of a particular batch, which is performed without DMSO.
I hope this information is helpful and wish you good luck with your research.
Answered on Aug 06 2012