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Inquiry: I have a question regarding ab109905. The detailed protocol booklet only covers the use of ab109905 for screening purposes using control bovine heart mitochondria. Can the kit method be adapted to use mitochondria isolated from animals treated with a potential complex III inhibitor? I have rat liver mitochondria which I would like to assay.... This work is likely to be published... in which case I would quote this product in print. If you could get back to me ASAP I would very much appreciate your assistance. Regards,
Asked on Mar 05 2013
Thank you for contacting us.
This assay is designed to measure the effect of a compound on a robust activity of BHM (which is provided with the kit). In case of other whole mitochondrial samples the user should optimize the assay themselves. The amount of mitochondria needed per test compound should also be optimized before testing the compounds.
The kit should work with any species, however the quality of the mitos will be very important.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Mar 05 2013