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more details about sample preparation step:
- which lysis buffer to be used? RIPA, TX-100 or NP40 or no detergent at all?
- can samples be freeze-thawed
will use whole cell lysate (not a mito prep)
Asked on Jul 26 2013
The MitoTox complex III oxphos assay does not require a detergent. The whole bovine mitochondria provided with the kit must be run according to the protocol (no further preparation required, simply dilute in the buffer provided).
The kit requires a mitochondria preparation if the customer is not going to use it with the sample provided. It will not work on whole cells or whole cell extract. The customer must make a mito preparation from whole cells. They could use kit https://www.abcam.com/mitochondria-isolation-kit-for-cultured-cells-with-dounce-homogenizer-ab110171.html to make whole mitos from cells.
This assay is an in-solution assay (no antibody included in the kit) and therefore it can work with mitochondria preparations from any species.
Sybille RexAbcam Scientific Support
Answered on Jul 26 2013