Anti-MK-3 antibody (ab155178)
Key features and details
- Rabbit polyclonal to MK-3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MK-3 antibody
See all MK-3 primary antibodies -
Description
Rabbit polyclonal to MK-3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human MK-3 aa 89-382 (C terminal).
Database link: Q16644 -
Positive control
- H1299, HeLaS3 and Mouse spleen lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression. -
Tissue specificity
Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313. -
Cellular localization
Nucleus. Cytoplasm. Predominantly located in the nucleus, when activated it translocates to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 615215 Cow
- Entrez Gene: 7867 Human
- Entrez Gene: 102626 Mouse
- Entrez Gene: 315994 Rat
- Omim: 602130 Human
- SwissProt: Q3SYZ2 Cow
- SwissProt: Q16644 Human
- SwissProt: Q3UMW7 Mouse
see all -
Alternative names
- 3pK antibody
- anti MAPKAPK3 antibody
- Chromosome 3p kinase antibody
see all
Images
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All lanes : Anti-MK-3 antibody (ab155178) at 1/5000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : MK-3 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 43 kDa -
All lanes : Anti-MK-3 antibody (ab155178) at 1/3000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
10% SDS PAGE -
Anti-MK-3 antibody (ab155178) at 1/10000 dilution + Mouse spleen whole cell lysate at 50 µg
Predicted band size: 43 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling MK-3 with ab155178 at 1/200 dilution (green). Lower image shows merge with DNA probe (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155178 has not yet been referenced specifically in any publications.