Overview

  • Product name

    Anti-MK-3 antibody [EPR13969]
    See all MK-3 primary antibodies
  • Description

    Rabbit monoclonal [EPR13969] to MK-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MK-3 aa 1-100. The exact sequence is proprietary.
    Database link: Q16644

  • Positive control

    • HeLa and Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13969
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/40 - 1/60.

Target

  • Function

    Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
  • Tissue specificity

    Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly located in the nucleus, when activated it translocates to the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3pK antibody
    • anti MAPKAPK3 antibody
    • Chromosome 3p kinase antibody
    • EC 2.7.11.1 antibody
    • MAP kinase-activated protein kinase 3 antibody
    • MAPK activated protein kinase 3 antibody
    • MAPK-activated protein kinase 3 antibody
    • MAPK3_HUMAN antibody
    • MAPKAP antibody
    • MAPKAP kinase 3 antibody
    • MAPKAP-K3 antibody
    • MAPKAP3 antibody
    • MAPKAPK 3 antibody
    • MAPKAPK-3 antibody
    • Mapkapk3 antibody
    • MAPKAPK3 antibody antibody
    • Mitogen activated protein kinase activated protein kinase 3 antibody
    • Mitogen activated protein kinase activated protein kinase antibody
    • MK-3 antibody
    see all

Images

  • Immunoprecipitation analysis of Jurkat cells labeling MK-3 using ab183040 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

  • Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling MK-3 with ab183040 at 1/100 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink).  Rabbit monoclonal IgG was used as Isotype control (green).

  • Anti-MK-3 antibody [EPR13969] (ab183040) at 1/5000 dilution + Jurkat cell line lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 43 kDa

  • Anti-MK-3 antibody [EPR13969] (ab183040) at 1/20000 dilution + HeLa cell line lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

References

This product has been referenced in:

  • Reynoird N  et al. Coordination of stress signals by the lysine methyltransferase SMYD2 promotes pancreatic cancer. Genes Dev 30:772-85 (2016). Read more (PubMed: 26988419) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183040.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up