Product nameAnti-MK2 antibody [E341]
See all MK2 primary antibodies
DescriptionRabbit monoclonal [E341] to MK2
Specificityab32567 recognises MAPKAP Kinase-2.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human MK2 aa 350-450 (C terminal). The exact sequence is proprietary.
- HeLa whole cell lysate (ab150035).
This product was previously labelled as MAPKAP Kinase 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).|
FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
Tissue specificityExpressed in all tissues examined.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
modificationsPhosphorylated and activated by MAP kinase.
- Information by UniProt
- MAP kinase activated protein Kinase 2 antibody
- MAP kinase-activated protein kinase 2 antibody
- MAPK activated protein kinase 2 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Anti-MK2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate
Predicted band size: 46 kDa
Observed band size: 46 kDa
Lanes 1-2 : Anti-MK2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
Lanes 3-4 : Anti-MK2 antibody [E341] (ab32567) at 1/500 dilution
Lanes 1 & 3 : UV treated HeLa cells
Lanes 2 & 4 : UV treated HeLa cells + p38 inhibitor
All lanes : IRDye® 680 LICOR goat anti-rabbit.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible cross reactivity)
Exposure time: 1 minute
ab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).