Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MK2 antibody [E341] (ab32567)

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Western blot - Anti-MK2 antibody [E341] (ab32567)
  • Western blot - Anti-MK2 antibody [E341] (ab32567)
  • Western blot - Anti-MK2 antibody [E341] (ab32567)
  • Anti-MK2 antibody [E341] (ab32567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E341] to MK2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-MK2 antibody [E341]
    See all MK2 primary antibodies

  • Description

    Rabbit monoclonal [E341] to MK2

  • Host species

    Rabbit

  • Specificity

    ab32567 recognises MAPKAP Kinase-2.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human MK2 aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Notes
WB
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC or IHC.

Target

  • Function

    Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.

  • Tissue specificity

    Expressed in all tissues examined.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated and activated by MAP kinase.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • MAP kinase activated protein Kinase 2 antibody
    • MAP kinase-activated protein kinase 2 antibody
    • MAPK activated protein kinase 2 antibody
    • MAPK-activated protein kinase 2 antibody
    • MAPK2_HUMAN antibody
    • MAPKAP K2 antibody
    • MAPKAP kinase 2 antibody
    • MAPKAPK 2 antibody
    • MAPKAPK-2 antibody
    • MAPKAPK2 antibody
    • Mitogen activated protein kinase activated protein kinase 2 antibody
    • MK 2 antibody
    • MK2 antibody
    see all

Images

  • Western blot - Anti-MK2 antibody [E341] (ab32567)
    Western blot - Anti-MK2 antibody [E341] (ab32567)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MK2 antibody [E341] (ab32567)
    Western blot - Anti-MK2 antibody [E341] (ab32567)
    Anti-MK2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Western blot - Anti-MK2 antibody [E341] (ab32567)
    Western blot - Anti-MK2 antibody [E341] (ab32567)
    Lanes 1-2 : Anti-MK2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
    Lanes 3-4 : Anti-MK2 antibody [E341] (ab32567) at 1/500 dilution

    Lanes 1 & 3 : UV treated HeLa cells
    Lanes 2 & 4 : UV treated HeLa cells + p38 inhibitor

    Secondary
    All lanes : IRDye® 680 LICOR goat anti-rabbit.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?
    Additional bands at: 55 kDa (possible cross reactivity)


    Exposure time: 1 minute


    ab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).

    See Abreview

  • Anti-MK2 antibody [E341] (ab32567)
    Anti-MK2 antibody [E341] (ab32567)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab32567? Please let us know so that we can cite the reference in this datasheet.

ab32567 has been referenced in 2 publications.

  • Muraleva NA  et al. SkQ1 Suppresses the p38 MAPK Signaling Pathway Involved in Alzheimer's Disease-Like Pathology in OXYS Rats. Antioxidants (Basel) 9:N/A (2020). PubMed: 32731533
  • McCaskill JL  et al. Broad-Spectrum Inhibition of Respiratory Virus Infection by MicroRNA Mimics Targeting p38 MAPK Signaling. Mol Ther Nucleic Acids 7:256-266 (2017). Dog, Human . PubMed: 28624201

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Western blot abreview for Anti-MK2 antibody [E341]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
35 µg
Treatment
UV treated samples (20 J dose)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Aug 20 2012

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