Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)

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Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)
  • Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)
  • Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E341] to MK2 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MK2 antibody [E341] - BSA and Azide free
    See all MK2 primary antibodies

  • Description

    Rabbit monoclonal [E341] to MK2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises MAPKAP Kinase-2. 

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247272 is the carrier-free version of ab32567 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247272 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as MAPKAP Kinase 2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC.

    • Target

    • Function

      Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.

    • Tissue specificity

      Expressed in all tissues examined.

    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Phosphorylated and activated by MAP kinase.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • MAP kinase activated protein Kinase 2 antibody
      • MAP kinase-activated protein kinase 2 antibody
      • MAPK activated protein kinase 2 antibody
      • MAPK-activated protein kinase 2 antibody
      • MAPK2_HUMAN antibody
      • MAPKAP K2 antibody
      • MAPKAP kinase 2 antibody
      • MAPKAPK 2 antibody
      • MAPKAPK-2 antibody
      • MAPKAPK2 antibody
      • Mitogen activated protein kinase activated protein kinase 2 antibody
      • MK 2 antibody
      • MK2 antibody
      see all

    Images

    • Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)
      Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)

      This data was developed using ab32567, the same antibody clone in a different buffer formulation.

      Lane 1: Wild-type HAP1 cell lysate (20 µg)

      Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg) 

      Lane 3: HeLa cell lysate (20 µg)

      Lane 4: A431 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

       

      ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)
      Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)
      Anti-MK2 antibody [E341] (ab32567) at 1/1000 dilution + HeLa cell lysate

      Predicted band size: 46 kDa
      Observed band size: 46 kDa



      This data was developed using ab32567, the same antibody clone in a different buffer formulation.

    • Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)
      Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247272? Please let us know so that we can cite the reference in this datasheet.

    ab247272 has not yet been referenced specifically in any publications.

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