Recombinant Anti-MK2 antibody [E341] - BSA and Azide free (ab247272)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MK2 antibody [E341] - BSA and Azide free
See all MK2 primary antibodies -
Description
Rabbit monoclonal [E341] to MK2 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody recognises MAPKAP Kinase-2.
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Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MK2 aa 350-450 (C terminal). The exact sequence is proprietary.
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General notes
ab247272 is the carrier-free version of ab32567 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247272 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as MAPKAP Kinase 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E341 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity. -
Tissue specificity
Expressed in all tissues examined. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated and activated by MAP kinase. - Information by UniProt
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Database links
- Entrez Gene: 9261 Human
- Entrez Gene: 17164 Mouse
- Entrez Gene: 289014 Rat
- Omim: 602006 Human
- SwissProt: P49137 Human
- SwissProt: P49138 Mouse
- Unigene: 643566 Human
- Unigene: 713747 Human
see all -
Alternative names
- MAP kinase activated protein Kinase 2 antibody
- MAP kinase-activated protein kinase 2 antibody
- MAPK activated protein kinase 2 antibody
see all
Datasheets and documents
References
ab247272 has not yet been referenced specifically in any publications.
