Overview

  • Product name

    Anti-MK2 (phospho T334) antibody
    See all MK2 primary antibodies
  • Description

    Rabbit polyclonal to MK2 (phospho T334)
  • Host species

    Rabbit
  • Specificity

    ab131504 detects endogenous levels of MK2 only when phosphorylated at threonine 334. The immunogen sequence used to produce ab131504 is 90% identical to the corresponding region of MAPKAP Kinase 3MK3, therefore ab131504 may cross react with human MK3 but this has not been confirmed.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MK2 conjugated to keyhole limpet haemocyanin.
    Sequence: PQTpPL
    Database link: NP_004750.1

  • Positive control

    • HeLa cells; HeLa cells treated with UV; Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as MAPKAP Kinase 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab131504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
  • Tissue specificity

    Expressed in all tissues examined.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated and activated by MAP kinase.
  • Information by UniProt
  • Database links

  • Alternative names

    • MAP kinase activated protein Kinase 2 antibody
    • MAP kinase-activated protein kinase 2 antibody
    • MAPK activated protein kinase 2 antibody
    • MAPK-activated protein kinase 2 antibody
    • MAPK2_HUMAN antibody
    • MAPKAP K2 antibody
    • MAPKAP kinase 2 antibody
    • MAPKAPK 2 antibody
    • MAPKAPK-2 antibody
    • MAPKAPK2 antibody
    • Mitogen activated protein kinase activated protein kinase 2 antibody
    • MK 2 antibody
    • MK2 antibody
    see all

Images

  • All lanes : Anti-MK2 (phospho T334) antibody (ab131504) at 1/500 dilution

    Lane 1 : Untreated HeLa cell extract
    Lane 2 : HeLa cell extract treated with UV

    Predicted band size: 46 kDa

  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling MK2 (phospho T334) with ab131504 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling MK2 (phospho T334) with ab131504 at 1/50 dilution (left) or ab131504 preincubated with blocking peptide (right).

References

This product has been referenced in:

  • Yang T  et al. lncRNA CCAT1 promotes cell proliferation, migration, and invasion by down-regulation of miR-143 in FTC-133 thyroid carcinoma cell line. Braz J Med Biol Res 51:e7046 (2018). Read more (PubMed: 29791590) »
  • Wang N  et al. Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF-a pathway. Int J Mol Med 42:1909-1916 (2018). Read more (PubMed: 30066830) »
See all 2 Publications for this product

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There are currently no Customer reviews or Questions for ab131504.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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