Key features and details
- Rabbit polyclonal to MK2 (phospho T334)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MK2 (phospho T334) antibody
See all MK2 primary antibodies
DescriptionRabbit polyclonal to MK2 (phospho T334)
Detects endogenous levels of MK2 only when phosphorylated at threonine 334 (human) and threonine 320 (mouse and rat). The immunogen sequence used to produce ab63378 is 90% identical to the corresponding region of MK3, therefore ab63378 may cross react with human MK3 but this has not been confirmed.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
This product was previously labelled as MAPKAP Kinase 2
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab63378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionIts physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
Tissue specificityExpressed in all tissues examined.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
modificationsPhosphorylated and activated by MAP kinase.
- Information by UniProt
- MAP kinase activated protein Kinase 2 antibody
- MAP kinase-activated protein kinase 2 antibody
- MAPK activated protein kinase 2 antibody
All lanes : Anti-MK2 (phospho T334) antibody (ab63378) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelled with ab63378 at 1/50 dilution. Right hand panel was treated with immunising phosphopeptide.
ICC/IF image of ab63378 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63378, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab63378 has been referenced in 5 publications.
- Cheng Z & Xing D Ginsenoside Rg3 inhibits growth and epithelial-mesenchymal transition of human oral squamous carcinoma cells by down-regulating miR-221. Eur J Pharmacol 853:353-363 (2019). PubMed: 30928631
- Li H et al. p38 MAPK-MK2 pathway regulates the heat-stress-induced accumulation of reactive oxygen species that mediates apoptotic cell death in glial cells. Oncol Lett 15:775-782 (2018). PubMed: 29387240
- Wu Y et al. Therapeutic Effect of MK2 Inhibitor on Experimental Murine Dry Eye. Invest Ophthalmol Vis Sci 58:4898-4907 (2017). PubMed: 28973340
- Zhai W et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016). Rat . PubMed: 27301321
- Lv L et al. WT1-AS promotes cell apoptosis in hepatocellular carcinoma through down-regulating of WT1. J Exp Clin Cancer Res 34:119 (2015). Human . PubMed: 26462627