Overview

  • Product name

    Anti-MK2 (phospho T334) antibody
    See all MK2 primary antibodies
  • Description

    Rabbit polyclonal to MK2 (phospho T334)
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of MK2 only when phosphorylated at threonine 334 (human) and threonine 320 (mouse and rat). The immunogen sequence used to produce ab63378 is 90% identical to the corresponding region of MK3, therefore ab63378 may cross react with human MK3 but this has not been confirmed.

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Human MK2.

    Synthetic peptide corresponding to Human MK2 (phospho T334).
    Sequence:

    PQTpPL

  • Positive control

    • Human breast carcinoma tissue NIH/3T3 cell extract
  • General notes

     This product was previously labelled as MAPKAP Kinase 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab63378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • Function

    Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
  • Tissue specificity

    Expressed in all tissues examined.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated and activated by MAP kinase.
  • Information by UniProt
  • Database links

  • Alternative names

    • MAP kinase activated protein Kinase 2 antibody
    • MAP kinase-activated protein kinase 2 antibody
    • MAPK activated protein kinase 2 antibody
    • MAPK-activated protein kinase 2 antibody
    • MAPK2_HUMAN antibody
    • MAPKAP K2 antibody
    • MAPKAP kinase 2 antibody
    • MAPKAPK 2 antibody
    • MAPKAPK-2 antibody
    • MAPKAPK2 antibody
    • Mitogen activated protein kinase activated protein kinase 2 antibody
    • MK 2 antibody
    • MK2 antibody
    see all

Images

  • All lanes : Anti-MK2 (phospho T334) antibody (ab63378) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa
    Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelled with ab63378 at 1/50 dilution. Right hand panel was treated with immunising phosphopeptide.
  • ICC/IF image of ab63378 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63378, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Cheng Z & Xing D Ginsenoside Rg3 inhibits growth and epithelial-mesenchymal transition of human oral squamous carcinoma cells by down-regulating miR-221. Eur J Pharmacol 853:353-363 (2019). Read more (PubMed: 30928631) »
  • Li H  et al. p38 MAPK-MK2 pathway regulates the heat-stress-induced accumulation of reactive oxygen species that mediates apoptotic cell death in glial cells. Oncol Lett 15:775-782 (2018). Read more (PubMed: 29387240) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
35 µg
Treatment
Irradiated with UV light (20 Joules) and cells were collected 1 hour later
Specification
HEK293 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted May 06 2012

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