Overview

  • Product name

  • Description

    Rabbit polyclonal to MKK6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human MKK6 aa 1-334.
    Database link: P52564

  • Positive control

    • 293T, HeLa, HepG2 and MOLT4 whole cell lysates; HeLa cells; Human N87 xenograft tissue.
  • General notes

     This product was previously labelled as MEK6

     

Properties

Applications

Our Abpromise guarantee covers the use of ab154901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
  • Tissue specificity

    Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Weakly autophosphorylated.
    Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links

  • Form

    It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
  • Alternative names

    • Dual specificity mitogen activated protein kinase kinase 6 antibody
    • Dual specificity mitogen-activated protein kinase kinase 6 antibody
    • MAP kinase kinase 6 antibody
    • MAP2K6 antibody
    • MAPK/ERK kinase 6 antibody
    • MAPKK 6 antibody
    • MAPKK6 antibody
    • MEK 6 antibody
    • MEK6 antibody
    • Mitogen Activated Protein Kinase Kinase 6 antibody
    • MKK 6 antibody
    • MKK6 antibody
    • MP2K6_HUMAN antibody
    • PRKMK6 antibody
    • protein kinase antibody
    • Protein kinase mitogen activated kinase 6 antibody
    • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody
    • SAPK kinase 3 antibody
    • SAPKK-3 antibody
    • SAPKK3 antibody
    • SKK3 antibody
    • Stress-activated protein kinase kinase 3 antibody
    see all

Images

  • Anti-MKK6 antibody (ab154901) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 37 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling MKK6 with ab154901 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human N87 xenograft tissue labeling MKK6 with ab154901 at 1/500 dilution.

References

This product has been referenced in:

  • Tang B  et al. PEA15 promotes liver metastasis of colorectal cancer by upregulating the ERK/MAPK signaling pathway. Oncol Rep 41:43-56 (2019). Read more (PubMed: 30365128) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154901.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up