Product nameAnti-MKK6 antibody [EP557Y]
See all MKK6 primary antibodies
DescriptionRabbit monoclonal [EP557Y] to MKK6
Specificityab33866 recognises MKK6.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human MKK6 aa 1-100 (N terminal). The exact sequence is proprietary.
- HeLa whole cell lysate (ab150035)
A trial size is available to purchase for this antibody.
This product was previously labelled as MEK6
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab33866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).|
FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Tissue specificityIsoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
FormIt localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
- Dual specificity mitogen activated protein kinase kinase 6 antibody
- Dual specificity mitogen-activated protein kinase kinase 6 antibody
- MAP kinase kinase 6 antibody
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab33866 was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Anti-MKK6 antibody [EP557Y] (ab33866) at 1/10000 dilution + Hela cell lysate
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?