Anti-MKK6 antibody [EP557Y] (ab33866)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-MKK6 antibody [EP557Y]
See all MKK6 primary antibodies -
DescriptionRabbit monoclonal [EP557Y] to MKK6
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Host speciesRabbit
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Specificityab33866 recognises MKK6.
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Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human MKK6 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- HeLa whole cell lysate (ab150035)
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General notes
A trial size is available to purchase for this antibody.
This product was previously labelled as MEK6
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberEP557Y
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab33866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa). | |
| IP | 1/50. |
Target
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FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
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Tissue specificityIsoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
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Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsWeakly autophosphorylated.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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Database links
- Entrez Gene: 5608 Human
- Entrez Gene: 26399 Mouse
- Entrez Gene: 114495 Rat
- Omim: 601254 Human
- SwissProt: P52564 Human
- SwissProt: P70236 Mouse
- Unigene: 463978 Human
- Unigene: 14487 Mouse
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FormIt localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
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Alternative names
- Dual specificity mitogen activated protein kinase kinase 6 antibody
- Dual specificity mitogen-activated protein kinase kinase 6 antibody
- MAP kinase kinase 6 antibody
see all
Images
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![Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)](https://www.abcam.com/ps/products/33/ab33866/Images/ab33866-251976-ab33866WBKO.jpg)
Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab33866 was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)](https://www.abcam.com/ps/products/33/ab33866/Images/ab33866-1.JPG)
Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)Anti-MKK6 antibody [EP557Y] (ab33866) at 1/10000 dilution + Hela cell lysate
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Datasheets and documents
References
This product has been referenced in:
- Grun D et al. NRP-1 interacts with GIPC1 and SYX to activate p38 MAPK signaling and cancer stem cell survival. Mol Carcinog N/A:N/A (2018). Read more (PubMed: 30456845) »
