Anti-MKK6 antibody [EP557Y] (ab33866)
![Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)](https://www.abcam.com/ps/products/33/ab33866/Images/ab33866-251976-anti-mek6-antibody-ep557y-western-blot.jpg "Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)")
Key features and details
- Rabbit monoclonal [EP557Y] to MKK6
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-MKK6 antibody [EP557Y]
See all MKK6 primary antibodies -
Description
Rabbit monoclonal [EP557Y] to MKK6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HepG2, C2C12, HeLa, HAP1, and C6 lysates IP: HepG2 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP557Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
|
|
| IP |
1/20 - 1/50.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa). |
|
IP
1/20 - 1/50. |
Target
-
Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively. -
Tissue specificity
Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsWeakly autophosphorylated.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
-
Database links
- Entrez Gene: 5608 Human
- Entrez Gene: 26399 Mouse
- Entrez Gene: 114495 Rat
- Omim: 601254 Human
- SwissProt: P52564 Human
- SwissProt: P70236 Mouse
- Unigene: 463978 Human
- Unigene: 14487 Mouse
-
Form
It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules. -
Alternative names
- Dual specificity mitogen activated protein kinase kinase 6 antibody
- Dual specificity mitogen-activated protein kinase kinase 6 antibody
- MAP kinase kinase 6 antibody
see all
Images
-
Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab33866 (unpurified) was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
-
![Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)](https://www.abcam.com/ps/products/33/ab33866/Images/ab33866-351358-anti-mkk6-antibody-ep557y-westernblot-human-mouse-rat.jpg)
Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)All lanes : Anti-MKK6 antibody [EP557Y] (ab33866) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-MKK6 antibody [EP557Y] (ab33866)](https://www.abcam.com/ps/products/33/ab33866/Images/ab33866-3-anti-mkk6-antibody-epr557y-immunoprecipitation-hepg2-human.jpg)
Immunoprecipitation - Anti-MKK6 antibody [EP557Y] (ab33866)ab33866 (purified) at 1/20 dilution (1 µg) immunoprecipitating MKK6 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab33866 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab33866 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (4)
ab33866 has been referenced in 4 publications.
- Norheim F et al. Genetic regulation of liver lipids in a mouse model of insulin resistance and hepatic steatosis. Mol Syst Biol 17:e9684 (2021). PubMed: 33417276
- Zhang X et al. MicroRNA-375 prevents TGF-ß-dependent transdifferentiation of lung fibroblasts via the MAP2K6/P38 pathway. Mol Med Rep 22:1803-1810 (2020). PubMed: 32582987
- Grun D et al. NRP-1 interacts with GIPC1 and SYX to activate p38 MAPK signaling and cancer stem cell survival. Mol Carcinog N/A:N/A (2018). PubMed: 30456845
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
