Overview

  • Product name

    Anti-MKK6 (phospho S207) antibody
    See all MKK6 primary antibodies
  • Description

    Rabbit polyclonal to MKK6 (phospho S207)
  • Host species

    Rabbit
  • Specificity

    ab131257 detects endogenous levels of MKK6 only when phosphorilated at Serine 207.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human MKK6 (phospho S207) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    VDS(p)VA


    Database link: P52564

  • Positive control

    • WB: HL60 cell extract treated with PMA. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as MEK6

     

Properties

Applications

Our Abpromise guarantee covers the use of ab131257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 37 kDa). Note: incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
  • Tissue specificity

    Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Weakly autophosphorylated.
    Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links

  • Form

    It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
  • Alternative names

    • Dual specificity mitogen activated protein kinase kinase 6 antibody
    • Dual specificity mitogen-activated protein kinase kinase 6 antibody
    • MAP kinase kinase 6 antibody
    • MAP2K6 antibody
    • MAPK/ERK kinase 6 antibody
    • MAPKK 6 antibody
    • MAPKK6 antibody
    • MEK 6 antibody
    • MEK6 antibody
    • Mitogen Activated Protein Kinase Kinase 6 antibody
    • MKK 6 antibody
    • MKK6 antibody
    • MP2K6_HUMAN antibody
    • PRKMK6 antibody
    • protein kinase antibody
    • Protein kinase mitogen activated kinase 6 antibody
    • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody
    • SAPK kinase 3 antibody
    • SAPKK-3 antibody
    • SAPKK3 antibody
    • SKK3 antibody
    • Stress-activated protein kinase kinase 3 antibody
    see all

Images

  • All lanes : Anti-MKK6 (phospho S207) antibody (ab131257) at 1/500 dilution

    Lane 1 : HL60 cell extract untreated
    Lane 2 : HL60 cell extract treated with PMA

    Predicted band size: 37 kDa

  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.

  • Immunofluorescence of methanol-fixed HeLa cells staining MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/100.

References

ab131257 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131257.
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