Anti-MKK6 (phospho S207) antibody (ab131257)
Key features and details
- Rabbit polyclonal to MKK6 (phospho S207)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MKK6 (phospho S207) antibody
See all MKK6 primary antibodies -
Description
Rabbit polyclonal to MKK6 (phospho S207) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human MKK6 (phospho S207) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P52564 -
Positive control
- WB: HL60 cell extract treated with PMA. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 37 kDa). Note: incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 37 kDa). Note: incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively. -
Tissue specificity
Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsWeakly autophosphorylated.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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Database links
- Entrez Gene: 5608 Human
- Entrez Gene: 26399 Mouse
- Omim: 601254 Human
- SwissProt: P52564 Human
- SwissProt: P70236 Mouse
- Unigene: 463978 Human
- Unigene: 14487 Mouse
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Form
It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules. -
Alternative names
- Dual specificity mitogen activated protein kinase kinase 6 antibody
- Dual specificity mitogen-activated protein kinase kinase 6 antibody
- MAP kinase kinase 6 antibody
see all
Images
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All lanes : Anti-MKK6 (phospho S207) antibody (ab131257) at 1/500 dilution
Lane 1 : HL60 cell extract untreated
Lane 2 : HL60 cell extract treated with PMA
Predicted band size: 37 kDa -
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
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Immunofluorescence of methanol-fixed HeLa cells staining MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131257 has not yet been referenced specifically in any publications.