• Product name

    Anti-MKK6 (phospho S207) antibody
    See all MKK6 primary antibodies
  • Description

    Rabbit polyclonal to MKK6 (phospho S207)
  • Host species

  • Specificity

    ab131257 detects endogenous levels of MKK6 only when phosphorilated at Serine 207.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human MKK6 (phospho S207) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.


    Database link: P52564

  • Positive control

    • WB: HL60 cell extract treated with PMA. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as MEK6




Our Abpromise guarantee covers the use of ab131257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 37 kDa). Note: incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.


  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
  • Tissue specificity

    Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Weakly autophosphorylated.
    Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links

  • Form

    It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
  • Alternative names

    • Dual specificity mitogen activated protein kinase kinase 6 antibody
    • Dual specificity mitogen-activated protein kinase kinase 6 antibody
    • MAP kinase kinase 6 antibody
    • MAP2K6 antibody
    • MAPK/ERK kinase 6 antibody
    • MAPKK 6 antibody
    • MAPKK6 antibody
    • MEK 6 antibody
    • MEK6 antibody
    • Mitogen Activated Protein Kinase Kinase 6 antibody
    • MKK 6 antibody
    • MKK6 antibody
    • MP2K6_HUMAN antibody
    • PRKMK6 antibody
    • protein kinase antibody
    • Protein kinase mitogen activated kinase 6 antibody
    • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody
    • SAPK kinase 3 antibody
    • SAPKK-3 antibody
    • SAPKK3 antibody
    • SKK3 antibody
    • Stress-activated protein kinase kinase 3 antibody
    see all


  • All lanes : Anti-MKK6 (phospho S207) antibody (ab131257) at 1/500 dilution

    Lane 1 : HL60 cell extract untreated
    Lane 2 : HL60 cell extract treated with PMA

    Predicted band size: 37 kDa

  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.

  • Immunofluorescence of methanol-fixed HeLa cells staining MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/100.


ab131257 has not yet been referenced specifically in any publications.

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