Key features and details
- Rabbit polyclonal to MKK6 (phospho S207)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-MKK6 (phospho S207) antibody
See all MKK6 primary antibodies
DescriptionRabbit polyclonal to MKK6 (phospho S207)
ab131257 detects endogenous levels of MKK6 only when phosphorilated at Serine 207.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- WB: HL60 cell extract treated with PMA. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma tissue.
This product was previously labelled as MEK6
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 37 kDa). Note: incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Tissue specificityIsoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
FormIt localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
- Dual specificity mitogen activated protein kinase kinase 6 antibody
- Dual specificity mitogen-activated protein kinase kinase 6 antibody
- MAP kinase kinase 6 antibody
All lanes : Anti-MKK6 (phospho S207) antibody (ab131257) at 1/500 dilution
Lane 1 : HL60 cell extract untreated
Lane 2 : HL60 cell extract treated with PMA
Predicted band size: 37 kDa
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
Immunofluorescence of methanol-fixed HeLa cells staining MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/100.
ab131257 has not yet been referenced specifically in any publications.