Overview

  • Product name

    Anti-MKK7 (phospho S271 + T275) antibody
    See all MKK7 primary antibodies
  • Description

    Rabbit polyclonal to MKK7 (phospho S271 + T275)
  • Host species

    Rabbit
  • Specificity

    This antibody does not react with other MEK isoforms.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human MKK7.
    (Peptide available as ab5742)

  • Positive control

    • Fusion protein expressing MEK 7.
  • General notes

     This product was previously labelled as MEK7

     

Properties

Applications

Our Abpromise guarantee covers the use of ab4762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 38 kDa.

Target

  • Function

    Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.
  • Tissue specificity

    Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Activated by phosphorylation on Ser/Thr.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • c-Jun N-terminal kinase kinase 2 antibody
    • Dual specificity mitogen activated protein kinase kinase 7 antibody
    • Dual specificity mitogen-activated protein kinase kinase 7 antibody
    • JNK activating kinase 2 antibody
    • JNK kinase 2 antibody
    • JNK-activating kinase 2 antibody
    • JNKK 2 antibody
    • Jnkk-2 antibody
    • Jnkk2 antibody
    • MAP kinase kinase 7 antibody
    • MAP2K7 antibody
    • MAPK/ERK kinase 7 antibody
    • MAPKK 7 antibody
    • MAPKK-7 antibody
    • MAPKK7 antibody
    • MEK 7 antibody
    • Mitogen Activated Protein Kinase kinase 7 antibody
    • MKK 7 antibody
    • MKK-7 antibody
    • MKK7 antibody
    • MP2K7_HUMAN antibody
    • PRKMK 7 antibody
    • PRKMK-7 antibody
    • PRKMK7 antibody
    • SAPK kinase 4 antibody
    • SAPKK-4 antibody
    • SAPKK4 antibody
    • Sek 2 antibody
    • Sek-2 antibody
    • Sek2 antibody
    • SKK4 antibody
    • stress-activated protein kinase kinase 4 antibody
    see all

Images

  • Peptide Competition: Extracts prepared from background extracts with tagged fusion protein expressing MEK 7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4762 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the

References

This product has been referenced in:

  • Xie SJ  et al. Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development. Cell Death Differ 25:1581-1597 (2018). Read more (PubMed: 29449644) »
  • Sheng X  et al. Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK. PLoS Pathog 13:e1006534 (2017). Read more (PubMed: 28753655) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Fibroblast)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
30 µg
Specification
Fibroblast
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 05 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up