Key features and details
- Rabbit polyclonal to MKKS
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MKKS antibody
DescriptionRabbit polyclonal to MKKS
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: RT4 and U-251 MG cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human urinary bladder, colon, kidney and testis tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 27 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProbable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. May play a role in protein processing in limb, cardiac and reproductive system development. May play a role in cytokinesis.
Tissue specificityWidely expressed in adult and fetal tissues.
Involvement in diseaseDefects in MKKS are the cause of McKusick-Kaufman syndrome (MKKS) [MIM:236700]. MKKS is an autosomal recessive developmental disorder. It is characterized by hydrometrocolpos, postaxial polydactyly and congenital heart defects.
Defects in MKKS are the cause of Bardet-Biedl syndrome type 6 (BBS6) [MIM:209900]. Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
DomainThe substrate-binding apical domain region is sufficient for centrosomal association.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytosol. The majority of the protein resides within the pericentriolar material (PCM), a proteinaceous tube surrounding centrioles. During interphase, the protein is confined to the lateral surfaces of the PCM but during mitosis it relocalizes throughout the PCM and is found at the intercellular bridge. The MKSS protein is highly mobile and rapidly shuttles between the cytosol and centrosome.
- Information by UniProt
- Bardet Biedl syndrome 6 protein antibody
- Bardet-Biedl syndrome 6 protein antibody
- BBS6 antibody
Paraffin-embedded human testis tissue stained for MKKS using ab254886 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for MKKS using ab254886 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human colon tissue stained for MKKS using ab254886 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human urinary bladder tissue stained for MKKS using ab254886 at 1/50 dilution in immunohistochemical analysis.
Lanes 2-3 : Anti-MKKS antibody (ab254886) at 0.4 µg/ml
Lane 1 : DNA Ladder
Lane 2 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 3 : U-251MG sp whole cell lysate
Predicted band size: 27 kDa
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling MKKS using ab254886 at 4µg/ml (green) in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254886 has not yet been referenced specifically in any publications.