The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use at an assay dependent concentration. PubMed: 23243284
Use a concentration of 1.25 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 99 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:1562500.
Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.
Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.
Involvement in disease
Note=A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
Contains 2 RPEL repeats. Contains 1 SAP domain.
The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity. The RPEL repeats mediate binding to globular actin.
Cytoplasm. Nucleus. Binding to globular actin is required to maintain cytoplasmic localization.
ICC/IF image of ab49311 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49311, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-Mkl1 antibody (ab49311)This image is courtesy of an anonymous Abreview.
Paraformaldehyde-fixed, 0.3% Triton X100 permeabilized HeLa (humanepithelial cell line from cervix adenocarcinoma) cells stained for Mkl1 (red) using ab49311 at 1/150 dilution in ICC/IF, followed by Goat anti Rabbit Cy3 secondary antibody at 1/100 dilution.
Ikeda T et al. Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs. Nat Commun9:1387 (2018).
Read more (PubMed: 29643333) »
Charles R et al. ARF GTPases control phenotypic switching of vascular smooth muscle cells through the regulation of actin function and actin dependent gene expression. Cell Signal46:64-75 (2018).
Read more (PubMed: 29499306) »