Anti-Mkl1/MRTFA antibody (ab113264)
Key features and details
- Rabbit polyclonal to Mkl1/MRTFA
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mkl1/MRTFA antibody
See all Mkl1/MRTFA primary antibodies -
Description
Rabbit polyclonal to Mkl1/MRTFA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Mkl1/MRTFA aa 700-800.
Database link: NP_065882.1 -
Positive control
- Human Breast Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation. -
Tissue specificity
Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain. -
Involvement in disease
Note=A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene. -
Sequence similarities
Contains 2 RPEL repeats.
Contains 1 SAP domain. -
Domain
The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.
The RPEL repeats mediate binding to globular actin. -
Cellular localization
Cytoplasm. Nucleus. Binding to globular actin is required to maintain cytoplasmic localization. - Information by UniProt
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Database links
- Entrez Gene: 57591 Human
- Omim: 606078 Human
- SwissProt: Q969V6 Human
- Unigene: 654688 Human
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Alternative names
- AI852829 antibody
- AMKL antibody
- AW743281 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (3)
ab113264 has been referenced in 3 publications.
- Rose S et al. Generation of proliferating human adult hepatocytes using optimized 3D culture conditions. Sci Rep 11:515 (2021). PubMed: 33436872
- Yamashiro C et al. Benzalkonium chloride-induced myofibroblastic transdifferentiation of Tenon's capsule fibroblasts is inhibited by coculture with corneal epithelial cells or by interleukin-10. Sci Rep 11:16096 (2021). PubMed: 34373467
- Kimura S et al. Tissue-scale tensional homeostasis in skin regulates structure and physiological function. Commun Biol 3:637 (2020). PubMed: 33127987