Anti-Mkln1 antibody (ab236986)
Key features and details
- Rabbit polyclonal to Mkln1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Mkln1 antibody
See all Mkln1 primary antibodies -
Description
Rabbit polyclonal to Mkln1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Orangutan -
Immunogen
Recombinant fragment corresponding to Human Mkln1 aa 450-650.
Database link: Q9UL63 -
Positive control
- WB: HeLa, HEK-293T and K562 cell lysate; Rat brain lysate. IHC-P: Human prostate tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000.
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IHC-P |
1/200 - 1/500.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. |
IHC-P
1/200 - 1/500. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component thrombospondin 1. -
Sequence similarities
Contains 1 CTLH domain.
Contains 6 Kelch repeats.
Contains 1 LisH domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4289 Human
- Entrez Gene: 27418 Mouse
- Entrez Gene: 83536 Rat
- Omim: 605623 Human
- SwissProt: Q9UL63 Human
- SwissProt: O89050 Mouse
- SwissProt: Q5RB35 Orangutan
- SwissProt: Q99PV3 Rat
see all -
Alternative names
- MKLN 1 antibody
- Mkln1 antibody
- MKLN1_HUMAN antibody
see all
Images
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Paraffin-embedded human prostate tissue stained for Mkln1 using ab236986 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4° overnight. The primary is detected by a biotinylated secondary and visualized using an HRP conjugated SP system.
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All lanes : Anti-Mkln1 antibody (ab236986) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : Rat brain lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution -
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mkln1 (green) using ab236986 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubate with primary antibody at 4°C overnight.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236986 has not yet been referenced specifically in any publications.