Key features and details
- Rabbit polyclonal to Mkln1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Mkln1 antibody
See all Mkln1 primary antibodies
DescriptionRabbit polyclonal to Mkln1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Orangutan
- WB: HeLa, HEK-293T and K562 cell lysate; Rat brain lysate. IHC-P: Human prostate tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|IHC-P||1/200 - 1/500.|
|ICC/IF||1/50 - 1/200.|
FunctionActs as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component thrombospondin 1.
Sequence similaritiesContains 1 CTLH domain.
Contains 6 Kelch repeats.
Contains 1 LisH domain.
- Information by UniProt
- MKLN 1 antibody
- Mkln1 antibody
- MKLN1_HUMAN antibody
Paraffin-embedded human prostate tissue stained for Mkln1 using ab236986 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4° overnight. The primary is detected by a biotinylated secondary and visualized using an HRP conjugated SP system.
All lanes : Anti-Mkln1 antibody (ab236986) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : Rat brain lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mkln1 (green) using ab236986 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubate with primary antibody at 4°C overnight.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236986 has not yet been referenced specifically in any publications.