Product nameAnti-Mkln1 antibody
See all Mkln1 primary antibodies
DescriptionRabbit polyclonal to Mkln1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Orangutan
- WB: HeLa, HEK-293T and K562 cell lysate; Rat brain lysate. IHC-P: Human prostate tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|IHC-P||1/200 - 1/500.|
|ICC/IF||1/50 - 1/200.|
FunctionActs as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component thrombospondin 1.
Sequence similaritiesContains 1 CTLH domain.
Contains 6 Kelch repeats.
Contains 1 LisH domain.
- Information by UniProt
- MKLN 1 antibody
- Mkln1 antibody
- MKLN1_HUMAN antibody
Paraffin-embedded human prostate tissue stained for Mkln1 using ab236986 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4° overnight. The primary is detected by a biotinylated secondary and visualized using an HRP conjugated SP system.
All lanes : Anti-Mkln1 antibody (ab236986) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : Rat brain lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mkln1 (green) using ab236986 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubate with primary antibody at 4°C overnight.
ab236986 has not yet been referenced specifically in any publications.