Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13519] to Mkln1
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-Mkln1 antibody [EPR13519]
See all Mkln1 primary antibodies
DescriptionRabbit monoclonal [EPR13519] to Mkln1
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Mkln1 aa 650 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UL63
- Jurkat, A431, Y79 and 293T cell lysates; Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab178403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 85 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionActs as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component thrombospondin 1.
Sequence similaritiesContains 1 CTLH domain.
Contains 6 Kelch repeats.
Contains 1 LisH domain.
- Information by UniProt
- MKLN 1 antibody
- Mkln1 antibody
- MKLN1_HUMAN antibody
All lanes : Anti-Mkln1 antibody [EPR13519] (ab178403) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Y79 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 85 kDa
Flow cytometry analysis of permeabilized Jurkat cells labeling Mkln1 (red) with ab178403 at a 1/100 dilution, or negative control rabbit IgG (green)
Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling Mkln1 with ab178403 at 1/10 dilution.
ab178403 has not yet been referenced specifically in any publications.