Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13519] to Mkln1 - BSA and Azide free
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-Mkln1 antibody [EPR13519] - BSA and Azide free
See all Mkln1 primary antibodies
DescriptionRabbit monoclonal [EPR13519] to Mkln1 - BSA and Azide free
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Mkln1 aa 650 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UL63
Ab250033 is the carrier-free version of ab178403. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250033 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab250033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionActs as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component thrombospondin 1.
Sequence similaritiesContains 1 CTLH domain.
Contains 6 Kelch repeats.
Contains 1 LisH domain.
- Information by UniProt
- MKLN 1 antibody
- Mkln1 antibody
- MKLN1_HUMAN antibody
All lanes : Anti-Mkln1 antibody [EPR13519] (ab178403) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Y79 (Human retinoblastoma cell line) cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 85 kDa
This data was developed using ab178403, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab250033 has not yet been referenced specifically in any publications.