Anti-MKLP1 antibody (ab168964)
Key features and details
- Mouse polyclonal to MKLP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MKLP1 antibody
See all MKLP1 primary antibodies -
Description
Mouse polyclonal to MKLP1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to amino acids 1-856 of Human MKLP1 (NP_004847.2, Q02241-2)
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Positive control
- MKLP1 transfected 293T cell lysate; HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab168964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles. -
Sequence similarities
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. - Information by UniProt
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Database links
- Entrez Gene: 9493 Human
- Omim: 605064 Human
- SwissProt: Q02241 Human
- Unigene: 270845 Human
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Alternative names
- CHO 1 antibody
- CHO1 antibody
- KIF 23 antibody
see all
Images
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Western blot - Anti-MKLP1 antibody (ab168964)All lanes : Anti-MKLP1 antibody (ab168964) at 1 µg/ml
Lane 1 : MKLP1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 89 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody (ab168964)Immunofluorescence of paraformaldehyde-fixed HeLa cells labeling MKLP1 using ab168964 at 10 µg/ml.
Protocols
Datasheets and documents
References (1)
ab168964 has been referenced in 1 publication.
- Li XL et al. KIF23 Promotes Gastric Cancer by Stimulating Cell Proliferation. Dis Markers 2019:9751923 (2019). PubMed: 31007778
