• Product name

  • Description

    Rabbit polyclonal to MKLP1
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH - which represented a portion within the first 100 amino acids of the human Mitotic Kinesin-Like Protein-1 (GenBank PID 400264).



Our Abpromise guarantee covers the use of ab9259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent dilution.
WB 1/1000 - 1/10000.


  • Function

    Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles.
  • Sequence similarities

    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • Database links

  • Alternative names

    • CHO 1 antibody
    • CHO1 antibody
    • KIF 23 antibody
    • KIF23 antibody
    • KIF23_HUMAN antibody
    • Kinesin family member 23 antibody
    • Kinesin like 5 antibody
    • Kinesin like protein 5 antibody
    • Kinesin like protein KIF 23 antibody
    • Kinesin like protein KIF23 antibody
    • Kinesin-like protein 5 antibody
    • Kinesin-like protein KIF23 antibody
    • KNS L5 antibody
    • KNSL 5 antibody
    • KNSL5 antibody
    • Mitotic kinesin like 1 antibody
    • Mitotic kinesin like protein 1 antibody
    • Mitotic kinesin-like protein 1 antibody
    • MKLP 1 antibody
    see all


  • ICC/IF image of ab9259 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Mollova M  et al. Cardiomyocyte proliferation contributes to heart growth in young humans. Proc Natl Acad Sci U S A 110:1446-51 (2013). Read more (PubMed: 23302686) »
  • Lordier L  et al. Megakaryocyte endomitosis is a failure of late cytokinesis related to defects in the contractile ring and Rho/Rock signaling. Blood 112:3164-74 (2008). Read more (PubMed: 18684864) »
See all 2 Publications for this product

Customer reviews and Q&As


All the infomraiton we have for ab9259 is on the online datasheet accessible via www.abcam.com. Sellitto and Kuriyama (1988) worked with a monoclonal antibody. This rabbit polyclonal antibody was raised agianst a synthetic peptide, which represented a portion within the first 100 amino acids of the human Mitotic Kinesin-Like Protein-1 (GenBank PID 400264), conjugated to KLH.

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