Key features and details
- Rabbit polyclonal to MKLP1
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MKLP1 antibody
See all MKLP1 primary antibodies
DescriptionRabbit polyclonal to MKLP1
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide (Human) conjugated to KLH - which represented a portion within the first 100 amino acids of the human Mitotic Kinesin-Like Protein-1 (GenBank PID 400264).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
Our Abpromise guarantee covers the use of ab9259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use at an assay dependent dilution.|
|WB||1/1000 - 1/10000.|
FunctionPlus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles.
Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle.
- Information by UniProt
- CHO 1 antibody
- CHO1 antibody
- KIF 23 antibody
ICC/IF image of ab9259 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab9259 has been referenced in 2 publications.
- Mollova M et al. Cardiomyocyte proliferation contributes to heart growth in young humans. Proc Natl Acad Sci U S A 110:1446-51 (2013). PubMed: 23302686
- Lordier L et al. Megakaryocyte endomitosis is a failure of late cytokinesis related to defects in the contractile ring and Rho/Rock signaling. Blood 112:3164-74 (2008). PubMed: 18684864