Overview

  • Product name
    Anti-MKLP1 antibody [EPR10879]
    See all MKLP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10879] to MKLP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MKLP1 aa 800-900 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q02241

  • Positive control
    • HeLa, 293T and A549 cell lysates. A547 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/400.
WB 1/1000. Predicted molecular weight: 110 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles.
    • Sequence similarities
      Belongs to the kinesin-like protein family.
      Contains 1 kinesin-motor domain.
    • Cellular localization
      Nucleus. Cytoplasm > cytoskeleton > spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHO 1 antibody
      • CHO1 antibody
      • KIF 23 antibody
      • KIF23 antibody
      • KIF23_HUMAN antibody
      • Kinesin family member 23 antibody
      • Kinesin like 5 antibody
      • Kinesin like protein 5 antibody
      • Kinesin like protein KIF 23 antibody
      • Kinesin like protein KIF23 antibody
      • Kinesin-like protein 5 antibody
      • Kinesin-like protein KIF23 antibody
      • KNS L5 antibody
      • KNSL 5 antibody
      • KNSL5 antibody
      • Mitotic kinesin like 1 antibody
      • Mitotic kinesin like protein 1 antibody
      • Mitotic kinesin-like protein 1 antibody
      • MKLP 1 antibody
      see all

    Images

    • Immunofluorescence staining of HeLa cells with purified ab174304 at a working dilution of 1 in 400, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab174304 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

    • Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/5000 dilution (purified) + RAW264.7 cell lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 110 kDa
      Observed band size: 98 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/1000 dilution (purified) + Rat lung tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 110 kDa
      Observed band size: 98 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/20000 dilution (purified) + HeLa cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 110 kDa
      Observed band size: 98 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/5000 dilution (purified)

      Lane 1 : HEK293 cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 110 kDa
      Observed band size: 110 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 110 kDa
      Additional bands at: 98-110 kDa. We are unsure as to the identity of these extra bands.

    • Immunofluorescent analysis of A547 cells labeling MKLP1 with unpurified ab174304 at 1/100.

    References

    This product has been referenced in:
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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