Recombinant Anti-MKLP1 antibody [EPR10879] (Alexa Fluor® 647) (ab204478)


  • Product name

    Anti-MKLP1 antibody [EPR10879] (Alexa Fluor® 647)
    See all MKLP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10879] to MKLP1 (Alexa Fluor® 647)
  • Host species

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MKLP1 aa 800-900 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q02241

  • Positive control

    • ICC/IF: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab204478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).


  • Function

    Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles.
  • Sequence similarities

    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • Database links

  • Alternative names

    • CHO 1 antibody
    • CHO1 antibody
    • KIF 23 antibody
    • KIF23 antibody
    • KIF23_HUMAN antibody
    • Kinesin family member 23 antibody
    • Kinesin like 5 antibody
    • Kinesin like protein 5 antibody
    • Kinesin like protein KIF 23 antibody
    • Kinesin like protein KIF23 antibody
    • Kinesin-like protein 5 antibody
    • Kinesin-like protein KIF23 antibody
    • KNS L5 antibody
    • KNSL 5 antibody
    • KNSL5 antibody
    • Mitotic kinesin like 1 antibody
    • Mitotic kinesin like protein 1 antibody
    • Mitotic kinesin-like protein 1 antibody
    • MKLP 1 antibody
    see all


  • ab204478 staining MKLP1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204478 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).


This product has been referenced in:

  • Li Y  et al. microRNA-300/NAMPT regulates inflammatory responses through activation of AMPK/mTOR signaling pathway in neonatal sepsis. Biomed Pharmacother 108:271-279 (2018). Read more (PubMed: 30223098) »
See 1 Publication for this product

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