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    mklp1-antibody-epr10879-bsa-and-azide-free-ab240178.pdf

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RecombinantRabMAb

Recombinant Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)

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  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
  • Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
  • Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
  • Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10879] to MKLP1 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

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Overview

  • Product name

    Anti-MKLP1 antibody [EPR10879] - BSA and Azide free
    See all MKLP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10879] to MKLP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240178 is the carrier-free version of ab174304.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10879
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin-like
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Myocardial regeneration

Associated products

  • Alternative Versions

    • Anti-MKLP1 antibody [EPR10879] (ab174304)
    • Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (ab204478)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles.
  • Sequence similarities

    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton > spindle.
  • Target information above from: UniProt accession Q02241 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9493 Human
    • Entrez Gene: 71819 Mouse
    • Entrez Gene: 315740 Rat
    • Omim: 605064 Human
    • SwissProt: Q02241 Human
    • Unigene: 270845 Human
    • Alternative names

      • CHO 1 antibody
      • CHO1 antibody
      • KIF 23 antibody
      • KIF23 antibody
      • KIF23_HUMAN antibody
      • Kinesin family member 23 antibody
      • Kinesin like 5 antibody
      • Kinesin like protein 5 antibody
      • Kinesin like protein KIF 23 antibody
      • Kinesin like protein KIF23 antibody
      • Kinesin-like protein 5 antibody
      • Kinesin-like protein KIF23 antibody
      • KNS L5 antibody
      • KNSL 5 antibody
      • KNSL5 antibody
      • Mitotic kinesin like 1 antibody
      • Mitotic kinesin like protein 1 antibody
      • Mitotic kinesin-like protein 1 antibody
      • MKLP 1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
      Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)

      Immunofluorescence staining of HeLa cells with purified ab174304 at a working dilution of 1 in 400, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab174304 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174304).

    • Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
      Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)

      Immunofluorescent analysis of A547 cells labeling MKLP1 with unpurified ab174304 at 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174304).

    • Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
      Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)

      Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with purified ab174304 at a working dilution of 1 in 400, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab174304 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab174304).

    • Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
      Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab240178? Please let us know so that we can cite the reference in this datasheet.

    ab240178 has not yet been referenced specifically in any publications.

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