Overview

  • Product name
  • Description
    Goat polyclonal to MKP-1
  • Host species
    Goat
  • Specificity
    The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human MKP-1 aa 355-367 (C terminal). The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).
    Sequence:

    SYLQSPITTSPSC


    Database link: P28562

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Most of the commercially available BSA and dry milk are contaminated with bovine IgG (even some of the higher purity grades of BSA); this might cause high background problems when labelled anti-goat secondary is used. The secondary might cross-react with bovine IgG since goat is closely related to cow.

Target

  • Function
    Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
  • Tissue specificity
    Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CL 100 antibody
    • CL100 antibody
    • Dual Specificity Phosphatase 1 antibody
    • Dual specificity protein phosphatase 1 antibody
    • Dual specificity protein phosphatase hVH1 antibody
    • DUS1_HUMAN antibody
    • DUSP 1 antibody
    • Dusp1 antibody
    • HVH1 antibody
    • MAP kinase phosphatase 1 antibody
    • Mitogen-activated protein kinase phosphatase 1 antibody
    • MKP-1 antibody
    • MKP1 antibody
    • Protein tyrosine phosphatase CL100 antibody
    • Protein-tyrosine phosphatase CL100 antibody
    • PTPN10 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH1 antibody
    see all

Images

  • Endogenous MKP-1 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence Endogenous MKP-1 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

  • ab1351 (3.8 µg/ml) staining MKP-1 in paraffin-embedded Human prostate tissue. Steam antigen retrieval was performed with citrate buffer (pH 6).

References

This product has been referenced in:
  • Shen J  et al. DUSP1 inhibits cell proliferation, metastasis and invasion and angiogenesis in gallbladder cancer. Oncotarget 8:12133-12144 (2017). Read more (PubMed: 28129656) »
  • Delehouzé C  et al. CDK/CK1 inhibitors roscovitine and CR8 downregulate amplified MYCN in neuroblastoma cells. Oncogene N/A:N/A (2013). Human . Read more (PubMed: 24317512) »

See all 4 Publications for this product

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chicken Cell lysate - whole cell (Thrombocyte)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
2 µg
Specification
Thrombocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mrs. Katie Elliott

Verified customer

Submitted Sep 29 2016

Application
Immunocytochemistry
Sample
Human Cultured Cells (Glioblastoma cells (U87MG, LN18,..) (all human))
Permeabilization
Yes - Triton x
Specification
Glioblastoma cells (U87MG, LN18,..) (all human)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Matthias Dedobbeleer

Verified customer

Submitted May 04 2016

We recommend using a permeablization step with this product in IHC-P. Permeabilize with 0.25% Triton X-100 for 10 minutes at room temperature. Perform this step after antigen retrieval and before blocking.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (spinal cord homogenate)
Gel Running Conditions
Reduced Denaturing (10% SDS_PAGE)
Loading amount
20 µg
Treatment
sham or nerve transected
Specification
spinal cord homogenate
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 17 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (HAPI cell line)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
400000 cells
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Specification
HAPI cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (BV2 cell line)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
400000 cells
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Specification
BV2 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Microglia)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
400000 cells
Treatment
LPS 50 ng/mL or LPS 50 ng/mL +UO 10 uM
Specification
Primary Microglia
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 10 2008

Thank you for your enquiry. I apologize for the delay in answering your question due to the holidays. The protocol used to test this antibody is below. 35ug protein per lane was loaded and its concentration would have been 2.5mg/ml in SDS Page loa...

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Thank you for your enquiry. The Western on the datasheet is detecting endogenous DUSP1 in Hela. In order to further troubleshoot why this antibody is not giving you any signal, it would be helpful if you could fill out the form at the link I am i...

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Thank you for your enquiry and for your patience. The originator of this antibody has informed me that they only observed a single band in HeLa lysate and we have not had any other feedback yet regarding the use of ab1351. However, looking at the seque...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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