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Our Abpromise guarantee covers the use of ab1351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml.|
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Most of the commercially available BSA and dry milk are contaminated with bovine IgG (even some of the higher purity grades of BSA); this might cause high background problems when labelled anti-goat secondary is used. The secondary might cross-react with bovine IgG since goat is closely related to cow.
Endogenous MKP-1 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Endogenous MKP-1 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence
ab1351 (3.8 µg/ml) staining MKP-1 in paraffin-embedded Human prostate tissue. Steam antigen retrieval was performed with citrate buffer (pH 6).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"