Anti-MKP-1 antibody (ab138265)
Key features and details
- Rabbit polyclonal to MKP-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MKP-1 antibody
See all MKP-1 primary antibodies -
Description
Rabbit polyclonal to MKP-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MKP-1 (C terminal). Immunogen range = between aa 241-290
Database link: P28562 -
Positive control
- HepG2 whole cell lysate (ab7900)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab138265 was affinity purified from rabbit antiserum by immunogenic peptide affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 39 kDa.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 39 kDa. |
Target
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Function
Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'. -
Tissue specificity
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain. - Information by UniProt
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Database links
- Entrez Gene: 1843 Human
- Entrez Gene: 19252 Mouse
- Entrez Gene: 114856 Rat
- Omim: 600714 Human
- SwissProt: P28562 Human
- SwissProt: P28563 Mouse
- SwissProt: Q64623 Rat
- Unigene: 171695 Human
see all -
Alternative names
- CL 100 antibody
- CL100 antibody
- Dual Specificity Phosphatase 1 antibody
see all
Images
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Western blot - Anti-MKP-1 antibody (ab138265)Anti-MKP-1 antibody (ab138265) at 1/500 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 39 kDa
Datasheets and documents
References (1)
ab138265 has been referenced in 1 publication.
- Almeida-Pereira G et al. 17ß-Estradiol attenuates p38MAPK activity but not PKCa induced by angiotensin II in the brain. J Endocrinol 240:345-360 (2019). PubMed: 30508412
