Overview

  • Product name

  • Description

    Rabbit polyclonal to MKP-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human MKP-1 (C terminal). Immunogen range = between aa 241-290
    Database link: P28562

  • Positive control

    • HepG2 whole cell lysate (ab7900)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138265 was affinity purified from rabbit antiserum by immunogenic peptide affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
  • Tissue specificity

    Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • CL 100 antibody
    • CL100 antibody
    • Dual Specificity Phosphatase 1 antibody
    • Dual specificity protein phosphatase 1 antibody
    • Dual specificity protein phosphatase hVH1 antibody
    • DUS1_HUMAN antibody
    • DUSP 1 antibody
    • Dusp1 antibody
    • HVH1 antibody
    • MAP kinase phosphatase 1 antibody
    • Mitogen-activated protein kinase phosphatase 1 antibody
    • MKP-1 antibody
    • MKP1 antibody
    • Protein tyrosine phosphatase CL100 antibody
    • Protein-tyrosine phosphatase CL100 antibody
    • PTPN10 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH1 antibody
    see all

Images

  • Anti-MKP-1 antibody (ab138265) at 1/500 dilution + HepG2 cell lysate at 30 µg

    Predicted band size: 39 kDa

References

ab138265 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
33 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 03 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up