Key features and details
- Mouse monoclonal [OTI4F10] to MKP-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MKP-1 antibody [OTI4F10]
See all MKP-1 primary antibodies
DescriptionMouse monoclonal [OTI4F10] to MKP-1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant full length protein corresponding to Human MKP-1 aa 1-367. Produced in E.coli (NP_004408).
MVMEVGTLDAGGLRALLGERAAQCLLLDCRSFFAFNAGHIAGSVNVRFST IVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSAALDGAKRDG TLALAAGALCREARAAQVFFLKGGYEAFSASCPELCSKQSTPMGLSLPLS TSVPDSAESGCSSCSTPLYDQGGPVEILPFLYLGSAYHASRKDMLDALGI TALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKNA GGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFS FMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHST NSALSYLQSPITTSPSC
Database link: P28562
- WB: pCMV6-ENTRY MKP-1 cDNA transfected HEK-293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 39 kDa.|
FunctionDual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
Tissue specificityExpressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain.
- Information by UniProt
- CL 100 antibody
- CL100 antibody
- Dual Specificity Phosphatase 1 antibody
All lanes : Anti-MKP-1 antibody [OTI4F10] (ab236501) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY DUSP1 cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 39 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236501 has been referenced in 1 publication.
- Shen J et al. MiR-32-5p influences high glucose-induced cardiac fibroblast proliferation and phenotypic alteration by inhibiting DUSP1. BMC Mol Biol 20:21 (2019). PubMed: 31438862