Key features and details
- Goat polyclonal to MKRN1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MKRN1 antibody
See all MKRN1 primary antibodies
DescriptionGoat polyclonal to MKRN1
Specificityab123804 is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1).
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Dog, Pig
- MKRN1 transfected HEK293 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab123804 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab123804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
1 hour primary incubation is recommended for this product.
FunctionE3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 4 C3H1-type zinc fingers.
Contains 1 RING-type zinc finger.
modificationsAuto-ubiquitinated; which leads to proteasomal degradation.
- Information by UniProt
- E3 ubiquitin-protein ligase makorin-1 antibody
- FLJ21334 antibody
- HGNC:7112 antibody
All lanes : Anti-MKRN1 antibody (ab123804) at 1 µg/ml
Lane 1 : fetal mouse brain tissue lysate
Lane 2 : adult mouse brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 53 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa (possible isoform)
Lane 1 : Anti-MKRN1 antibody (ab123804) at 0.3 µg/ml
Lane 2 : Anti-MKRN1 antibody (ab123804) at 1 mg/ml
Lane 3 : anti-DYKDDDDK Tag anntibody at 1/1000 dilution
Lane 1 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
Lane 2 : HEK293 lysate, mock-transfected
Lane 3 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
ab123804 has not yet been referenced specifically in any publications.