• Product name

  • Description

    Rabbit polyclonal to MKRN1
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 432 and the C-terminus (residue 482) of human MKRN1 (NP_038474.1).

  • Positive control

    • Whole cell lysates from HeLa and 293T cells.



Our Abpromise guarantee covers the use of ab72054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
  • Tissue specificity

  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • Post-translational

    Auto-ubiquitinated; which leads to proteasomal degradation.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase makorin-1 antibody
    • FLJ21334 antibody
    • HGNC:7112 antibody
    • Makorin antibody
    • Makorin ring finger protein 1 antibody
    • MKRN 1 antibody
    • mkrn1 antibody
    • MKRN1_HUMAN antibody
    • OTTHUMP00000212117 antibody
    • OTTHUMP00000212118 antibody
    • OTTHUMP00000212121 antibody
    • OTTHUMP00000212124 antibody
    • Ring finger protein 1 antibody
    • RING finger protein 61 antibody
    • RNF61 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MKRN1 with ab72054 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MKRN1 antibody (ab72054) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 100 kDa, 16 kDa, 28 kDa, 45 kDa. We are unsure as to the identity of these extra bands.


This product has been referenced in:

  • Liu M  et al. Hypoxia-induced feedback of HIF-1a and lncRNA-CF129 contributes to pancreatic cancer progression through stabilization of p53 protein. Theranostics 9:4795-4810 (2019). Read more (PubMed: 31367258) »
  • Panneer Selvam S  et al. Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation. Sci Signal 8:ra58 (2015). WB ; Human . Read more (PubMed: 26082434) »
See all 3 Publications for this product

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