Key features and details
- Rabbit polyclonal to MKRN2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MKRN2 antibody
See all MKRN2 primary antibodies
DescriptionRabbit polyclonal to MKRN2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to a region between residue 366 and the C-terminus (residue 416) of human MKRN2 (NP_054879.3).
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 47 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceMKRN2 (Makorin 2) contains 4 C3H1 type zinc fingers.
- HSPC070 antibody
- Makorin 2 antibody
- Makorin ring finger protein 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MKRN2 with ab72055 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-MKRN2 antibody (ab72055) at 0.1 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from HeLa cells at 5 µg
Lane 4 : Whole cell
lysate from 293T cells at 50 µg
Lane 5 : Whole cell
lysate from NIH 3T3 cells at 50 µg
Predicted band size: 47 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Detection of Human MKRN2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72055 at 3 µg/mg lysate (Lane 1). Lane 2 represents Control IgG IP. Subsequent Western blot detection was done using ab72055 at 1 µg/ml.
ab72055 has not yet been referenced specifically in any publications.