Overview

  • Product name

    Anti-MKS1 antibody
  • Description

    Rabbit polyclonal to MKS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 150-251 of Human MKS1 (UniProt: Q9NXB0).

  • Positive control

    • Human colon tissue; RT4 lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 65 kDa.

Target

  • Function

    Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology.
  • Involvement in disease

    Meckel syndrome 1
    Bardet-Biedl syndrome 13
  • Sequence similarities

    Contains 1 B9 domain.
  • Cellular localization

    Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Localizes at the transition zone, a region between the basal body and the ciliary axoneme.
  • Information by UniProt
  • Database links

  • Alternative names

    • B8d3 antibody
    • BBS13 antibody
    • Dysencephalia splanchnocystica antibody
    • FABB proteome like protein antibody
    • FLJ20345 antibody
    • Gruber syndrome antibody
    • Meckel gruber syndrome antibody
    • Meckel gruber syndrome type 1 antibody
    • Meckel syndrome antibody
    • Meckel syndrome type 1 antibody
    • Meckel syndrome type 1 protein antibody
    • Meckel syndrome type 1 protein homolog antibody
    • MES antibody
    • MKS 1 antibody
    • MKS antibody
    • Mks1 antibody
    • MKS1_HUMAN antibody
    • POC12 antibody
    • POC12 centriolar protein homolog antibody
    see all

Images

  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling MKS1 with ab150807 antibody at a dilution of 1/50.
  • All lanes : Anti-MKS1 antibody (ab150807) at 1/100 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Predicted band size: 65 kDa

References

ab150807 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3)
Gel Running Conditions
Reduced Denaturing
Loading amount
55 µg
Treatment
4 µg DNA for 24hrs
Specification
NIH3T3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 13 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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