Overview

  • Product name

  • Description

    Rabbit polyclonal to MKX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Mouse MKX aa 181-230 (internal sequence).
    Sequence:

    EGYSTPAHHTVIKGESSAIKAGGRPESRAAEDYVSPPKYKSSLLNRYLND

  • Positive control

    • NIH 3T3 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    May act as a morphogenetic regulator of cell adhesion.
  • Sequence similarities

    Belongs to the TALE/IRO homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9430023B20Rik antibody
    • C10orf48 antibody
    • Homeobox protein Mohawk antibody
    • IFRX antibody
    • Iroquois family related homeodomain protein antibody
    • iroquois homeobox protein like 1 antibody
    • IRXL1 antibody
    • MGC39616 antibody
    • MKX antibody
    • MKX_HUMAN antibody
    • mohawk homeobox antibody
    • OTTHUMP00000019374 antibody
    see all

Images

  • Anti-MKX antibody (ab66939) at 2.5 µg/ml + NIH 3T3 lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa
    Additional bands at: ~75 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration 12%

References

ab66939 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Olfactory Stem Cells of Rabbit)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rabbit
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Antoine Veron

Verified customer

Submitted Jul 22 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory Stem Cells (GFP))
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells (GFP)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Antoine Veron

Verified customer

Submitted May 31 2016

Application
Western blot
Sample
Horse Cell lysate - whole cell (various cell type)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
various cell type
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 23 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells (tenocytes differentiation))
Permeabilization
Yes - 0,1 % Triton X100
Specification
Olfactory stem cells (tenocytes differentiation)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Antoine Veron

Verified customer

Submitted Aug 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells (differentiation tendon))
Permeabilization
Yes - 0,1% Triton 100X
Specification
Olfactory stem cells (differentiation tendon)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Antoine Veron

Verified customer

Submitted Jun 19 2015

Answer

Thank you for contacting us and your interest in our products.

As you have seen from our website, the anti-OAF antibody (ab116332) has not as yet been used in detecting themouse protein as you are hoping to do. This does not mean that it would not work, simply that we have not had any reports supporting it yet. As the immunogen used to raise this antibody (synthetic peptide taken from within residues 54-83 of human OAF)shares more than 85% homology with themouse protein we would therefore expect the antibody to work with mouse samples but as we have not tested it ourselves we would not be able to guarantee this.

Unfortunately we are not able to provide sample sizes of any of our products. This is due to the logistics that would be involved in this, with such a large catalogue this simply isn't feasible. However, we do offer a testing discount scheme. This offer means that if you purchase the ab116332 as usual, test the antibodywith your mousesamplesand share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.

We have one antibody which has been raised against mouse Mohawk homeobox protein (ab66939). This has currently only been used in Western blotting to detect the protein in NIH 3T3 lysates. Let me reassure you that if you are hoping to use the antibody for Western blotting the antibody would be covered by the Abpromise guarantee. This means that if the antibody does not perform as stated by the datasheet and the problem is reported within 6 months, you would be entitled to a free of charge replacement or a refund. More information on the Abpromise can be found from the following page:

https://www.abcam.com/abpromise

If you hope to use it for any other application I would also be able to offer you a testing discount.

I hope this information has been of help. If you require any further information please do not hesitate to ask.

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