Key features and details
- Mouse monoclonal [OTI6A12] to MKX
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MKX antibody [OTI6A12]
See all MKX primary antibodies
DescriptionMouse monoclonal [OTI6A12] to MKX
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Recombinant full length protein corresponding to Human MKX aa 1-352. Produced in E.coli (NP_775847).
MNTIVFNKLSGAVLFEDGGASERERGGRPYSGVLDSPHARPEVGIPDGPP LKDNLGLRHRRTGARQNGGKVRHKRQALQDMARPLKQWLYKHRDNPYPTK TEKILLALGSQMTLVQVSNWFANARRRLKNTVRQPDLSWALRIKLYNKYV QGNAERLSVSSDDSCSEDGENPPRTHMNEGGYNTPVHHPVIKSENSVIKA GVRPESRASEDYVAPPKYKSSLLNRYLNDSLRHVMATNTTMMGKTRQRNH SGSFSSNEFEEELVSPSSSETEGNFVYRTDTLENGSNKGESAANRKGPSK DDTYWKEINAAMALTNLAQGKDKLQGTTSCIIQKSSHIAEVKTVKVPLVQ QF
Database link: Q8IYA7
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY MKX cDNA for 48 hrs. IHC-P: Human prostate and prostate carcinoma tissues.
Clone OTI6A12 (formerly 6A12).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 minutes.
|WB||1/2000. Predicted molecular weight: 39 kDa.|
FunctionMay act as a morphogenetic regulator of cell adhesion.
Sequence similaritiesBelongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- 9430023B20Rik antibody
- C10orf48 antibody
- Homeobox protein Mohawk antibody
All lanes : Anti-MKX antibody [OTI6A12] (ab236400) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs.
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY MKX cDNA for 48 hrs.
Lysates/proteins at 5 µg per lane.
Predicted band size: 39 kDa
Paraffin-embedded human prostate tissue stained for MKX using ab236400 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate carcinoma tissue stained for MKX using ab236400 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236400 has not yet been referenced specifically in any publications.