Key features and details
- Rabbit polyclonal to MLC1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-MLC1 antibody
See all MLC1 primary antibodies
DescriptionRabbit polyclonal to MLC1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Chinese hamster
corresponding to MLC1.
(Peptide available as
- This antibody gave a positive signal in the following Mouse Tissue lysates: Brain; Forebrain; Cortex; Hippocampus; Cerebellum; Olfactory Bulb.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab130770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 42 kDa).|
FunctionRegulates the response of astrocytes to hypo-osmosis by promoting calcium influx.
Tissue specificityExpressed in the brain, with highest levels found in the amygdala, nucleus caudatus, thalamus and hippocampus.
Involvement in diseaseLeukoencephalopathy, megalencephalic, with subcortical cysts, 1
Cellular localizationMembrane. Cell membrane. Cytoplasm, perinuclear region. Endoplasmic reticulum.
- Information by UniProt
- KIAA0027 antibody
- LVM antibody
- Megalencephalic leukoencephalopathy with subcortical cysts 1 antibody
All lanes : Anti-MLC1 antibody (ab130770) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Mouse Olfactory Bulb Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 240 kDa, 27 kDa, 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab130770 detects a band at 44 + 77 kDa, while this differs to its predicted molecular weight of 42 kDa, the banding pattern observed is consistent with what has been described in the literature PMID: 15367490. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130770 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab130770 has been referenced in 1 publication.
- Hwang J et al. Plasma membrane localization of MLC1 regulates cellular morphology and motility. Mol Brain 12:116 (2019). PubMed: 31888684