Overview

  • Product name

  • Description

    Rabbit polyclonal to MLC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Chinese hamster
  • Immunogen

    corresponding to MLC1.
    (Peptide available as ab169568)

  • Positive control

    • This antibody gave a positive signal in the following Mouse Tissue lysates: Brain; Forebrain; Cortex; Hippocampus; Cerebellum; Olfactory Bulb.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 42 kDa).

Target

  • Function

    Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx.
  • Tissue specificity

    Expressed in the brain, with highest levels found in the amygdala, nucleus caudatus, thalamus and hippocampus.
  • Involvement in disease

    Leukoencephalopathy, megalencephalic, with subcortical cysts, 1
  • Cellular localization

    Membrane. Cell membrane. Cytoplasm, perinuclear region. Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0027 antibody
    • LVM antibody
    • Megalencephalic leukoencephalopathy with subcortical cysts 1 antibody
    • Membrane protein MLC1 antibody
    • MLC antibody
    • MLC1 antibody
    • MLC1_HUMAN antibody
    • VL antibody
    • WKL1 antibody
    see all

Images

  • All lanes : Anti-MLC1 antibody (ab130770) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Forebrain (Mouse) Tissue Lysate
    Lane 3 : Mouse Cortex Tissue Lysate
    Lane 4 : Mouse Hippocampus Tissue Lysate
    Lane 5 : Cerebellum Mouse Tissue Lysate
    Lane 6 : Mouse Olfactory Bulb Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 240 kDa, 27 kDa, 77 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    ab130770 detects a band at 44 + 77 kDa, while this differs to its predicted molecular weight of 42 kDa, the banding pattern observed is consistent with what has been described in the literature PMID: 15367490. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130770 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab130770 has not yet been referenced specifically in any publications.

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