Western blot abreview for Anti-MLC1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (brain)
Loading amount
20 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat dry milk in 0.05% PBS-T

Secondary antibody

Dilution
1/5000

Detection

Detection method
ecl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 43 kDa

Additional data

Additional Notes
Very nice one clear band on expected size.
1. Whole brain lysate

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Submitted Jun 07 2012

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